Composite

Part:BBa_K1925006:Design

Designed by: Zi Yue Wang   Group: iGEM16_Fudan   (2016-10-11)


mitronic GFP backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 448
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cuz its complexity of sequence，PCR will be a little difficult


Source

De novo synthesis from company Genewiz

References